METHOD OF PRODUCTION OF BACTERIAL CONCENTRATE AND ITS APPLICATION AS BIOLOGICALLY ACTIVE FOOD SUPPLEMENT OR INOCULUM FOR DIRECT APPLICATION FOR KURUNGA Russian patent published in 2014 - IPC A23C9/12 A23C9/127 C12P1/00 

FIELD: biotechnology.

SUBSTANCE: method comprises growing symbiotic inoculum of kefir fungal inoculum and thermophilic lactic acid bacteria Lactobacillus bulgaricus, Lactobacillus acidophilus and Lactobacillus helveticus (1:0.5:0.5:1) on the nutrient medium based on cottage cheese whey, rye flour and growth components at 30±2°C for 8-10 hours. After receiving the inoculum the biomass is isolated to obtain the liquid bacterial concentrate. In another embodiment for preparation of the inoculum of direct application the resulting liquid bacterial concentrate is mixed with the protective medium and frozen at a temperature not higher than (-20°C). The use of the resulting concentrates is proposed as a biologically active food supplement or the inoculum of direct application for kurunga.

EFFECT: inventions enable to increase the biomass yield and to maintain stability of microflora of the bacterial concentrate during prolonged storage to obtain the biologically active food supplement or the inoculum of direct application.

4 cl, 8 tbl, 3 dwg, 6 ex