FIELD: chemistry.
SUBSTANCE: invention relates to a method of forming a 3-phenylimino-3H-phenothiazine mediator or a 3-phenylimino-3H-phenoxazine mediator, which includes providing a first reactant including phenothiazine or phenoxazine, providing a first solvent, providing a second reactant and providing a second solvent. The first reactant, first solvent, second reactant and second solvent are combined to form a reactant solution. Sodium persulphate is added to the reactant solution to link the first and second reactants resulting in a reaction solution containing the 3-phenylimino-3H-phenothiazine or the 3-phenylimino-3H-phenoxazine mediator. Further treatment of the reaction solution is carried out to remove the first solvent and second solvent in order to separate the mediators. The invention also relates to products obtained using said method and use thereof in an electrochemical test sensor.
EFFECT: disclosed mediators have a low background current and have the required characteristics, including stability.
12 cl, 1 tbl, 25 ex, 5 dwg
