FIELD: medicine.
SUBSTANCE: invention refers to molecular biology and gene therapy. The method provides carrying out the following procedures: cartridge construct designing, synthesis of oligonucleotides; pair-wise annealing and setting of partially complementary oligonucleotides; precipitation and restriction of the cartridge fragments with applicable restriction nucleases; simultaneous ligation of separate cartridge fragments into one linear molecule; two PCR amplifications, electrophoresis in agarose miniature gel and electroelution; ligation into pGemT-easy vector; transformation and selection of positive clones by means of colony-hybridisation; sequence analysis of a DNA of plasmid clones; re-cloning of the cartridges into an expression vector.
EFFECT: method enables producing the cartridge gene constructs expressing several shRNAs.
8 dwg, 1 ex
| Title |
Year |
Author |
Number |
| HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS |
2012 |
- Tsao Tszekhuej
- Novak Stefen
- Chzhou Nin
|
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2012 |
- Churikov Nikolaj Andreevich
- Kretova Ol'Ga Valer'Evna
|
RU2552607C2 |
| CARTRIDGE GENETIC MAKER EXPRESSING THREE BIOLOGICALLY ACTIVE siPHK, EFFECTIVELY ATTACKING HUMAN IMMUNODEFICIENCY VIRUS AND CCR5 GENE TRANSCRIPTS BY MEANS OF RNA-INTERFERENCE |
2009 |
- Churikov Nikolaj Andreevich
- Kretova Ol'Ga Valer'Evna
|
RU2425150C1 |
| CARTRIDGE GENETIC CONSTRUCT EXPRESSING TWO BIOLOGICALLY ACTIVE siRNAS EFFECTIVELY ATTACKING SUBTYPE A HIV-1 TRANSCRIPTS TARGETED TO iRNA OF REVERSE TRANSCRIPTASE AND INTEGRASE, AND ONE siRNA TARGETED TO iRNA OF CCR5 GENE IN RUSSIAN PATIENTS |
2013 |
- Churikov Nikolaj Andreevich
- Kretova Ol'Ga Valer'Evna
|
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2021 |
- Bochkareva Mariya Dmitrievna
- Imatdinov Ilnaz Ramisovich
- Imatdinov Almaz Ramisovich
- Sulgina Tatyana Vladimirovna
- Tishin Anton Evgenevich
- Sulgin Aleksandr Andreevich
- Prudnikova Elena Yurevna
- Gavrilova Elena Vasilevna
- Maksyutov Rinat Amirovich
|
RU2769125C1 |
| CASSETTE GENETIC CONSTRUCT EXPRESSING TWO BIOLOGICALLY ACTIVE SIRNA, EFFECTIVELY ATTACKING TARGETS IN REVERSE TRANSCRIPTASE MRNA OF SUBTYPE A HIV-1 IN RUSSIAN PATIENTS, AND ONE SIRNK TARGETED TO MRNA OF CCR5 GENE |
2015 |
- Churikov Nikolaj Andreevich
- Kretova Olga Valerevna
- Fedoseeva Darya Mikhajlovna
|
RU2607381C1 |
| RING DNA MOLECULE WITH CONDITION-DEPENDENT REPLICON ORIGIN, METHOD FOR PRODUCTION THEREOF AND USES IN GENE THERAPY |
2003 |
|
RU2307870C2 |
| CARTRIDGE GENETIC CONSTRUCT EXPRESSING TWO BIOLOGICALLY ACTIVE SIRNAS EFFECTIVELY ATTACKING TARGETS IN MRNA OF VPU AND ENV GENES OF HIV-1 SUBTYPE A IN RUSSIAN PATIENTS, AND ONE SIRNA AIMED AT CCR5 GENE MRNA |
2016 |
- Churikov Nikolaj Andreevich
- Kretova Olga Valerevna
|
RU2630644C1 |
| GENETIC CONSTRUCT FOR GENE EXPRESSION mNG_CD4-CXCR4, RECOMBINANT PLASMID rVSV_MNG_CD4-CXCR4 AND A RECOMBINANT VESICULAR STOMATITIS VIRUS STRAIN rVSV_mNG_CD4-CXCR4, WHICH PROVIDES TARGETED VIROLYSIS OF CELLS EXPOSING HIV-1 GP120/GP41 PROTEINS TROPISM X4 ON THEIR SURFACE |
2021 |
- Bochkareva Mariya Dmitrievna
- Imatdinov Ilnaz Ramisovich
- Imatdinov Almaz Ramisovich
- Sulgina Tatyana Vladimirovna
- Tishin Anton Evgenevich
- Sulgin Aleksandr Andreevich
- Prudnikova Elena Yurevna
- Gavrilova Elena Vasilevna
- Maksyutov Rinat Amirovich
|
RU2768032C1 |
| ANTITEMPLATE PLASMID VECTOR pBL-T FOR CLONING OF COMPLEX GENOME SEQUENCES AND MULTIMODULE ASSEMBLY OF GENETICALLY ENGINEERED CONSTRUCTS (VERSIONS), AND SET CONTAINING THIS VECTOR |
2011 |
- Orlova Nadezhda Aleksandrovna
- Vorob'Ev Ivan Ivanovich
- Zvjagin Ivan Vladimirovich
- Shender Viktorija Olegovna
|
RU2507266C2 |
