METHOD OF DIFFERENTIAL MORPHOMETRIC DIAGNOSTICS OF ERYTHRODERMAL FORM OF MYCOSIS FUNGOIDES AND SYNDROME OF SKIN PSEUDOLYMPHOMA BY RELATIVE VOLUME OF EPIDERMIS AND MITOTIC INDEX OF EPIDERMAL CELLS Russian patent published in 2014 - IPC G01N33/483 G01N33/53 

FIELD: medicine.

SUBSTANCE: to perform differential morphometric diagnostics of an erythrodermal form of mycosis fungoides and the syndrome of skin pseudolymphom performed are: histological analysis of the affected skin biopsy samples by a method of light microscopy, immunodifferentiation of infiltrate cells, additional morphometric analysis of basal keratinocytes in histological preparations of the affected skin biopsy samples. In case of the similar histological picture, described in case of erythrodermal form of mycosis fungoides and the syndrome of skin pseudolymphoma such as acanthosis, hyperkeratosis, parakeratosis, oedema and vasodilatation of the derma, lymphohistiocytic infiltrates, located in the upper third of the derma, similar immunophenotype of the infiltrate cells in case of erythrodermal form of mycosis fungoides and the syndrome of skin pseudolymphoma, represented by CD2+, CD3+, CD4+, CD5+, CD43+, CD45RO+ lymphocytes, morphometric analysis of all epidermis cells is performed. After that, the relative volume of epidermis and mitotic index of the epidermal cells are calculated. In case of increase of the relative volume of the epidermis by 2.2 and more times in comparison with the normal skin and an increase of the mitotic index of the epidermal cells by 2.7 times and more in comparison with the normal skin, erythrodermal form of mycosis fungoides is diagnosed, and in case of the unchanged relative volume of the epidermis and the mitotic index of the epidermal cells in comparison with the normal skin, the syndrome of skin pseudolymphoma is diagnosed.

EFFECT: acceleration and accuracy of differential diagnostics of the said forms of skin lymphomas, which makes it possible to select tactics of early adequate rational treatment of the patient.

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