FIELD: medicine.
SUBSTANCE: invention concerns a method for Staphylococcus aureus genotyping. The presented method involves preparing a pure culture on a solid nutrient medium with DNA purification and amplification by multiplex polymerase chain reaction (PCR) and result detection by agarose gel electrophoresis. The multiplex PCR involves using four pairs of primers complementary to extracellular thermonuclease (nuc) gene, Panton-Valentine leukocidin (pvl) gene, toxic shock protein (tst) gene and methicillin resistance (mecA) gene sites. Staphylococcus aureus is genotyped by the presence or absence of pvl and tst virulence genes and mecA methicillin resistance genes or combinations thereof.
EFFECT: invention enables Stafylococcus aureus genotyping in a combination with the multiplex reaction, as well as provides eliminating the preliminary stage of microorganism identification.
1 dwg, 2 tbl, 2 ex
