FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology, namely to molecular biology. A set of oligonucleotide primers and a fluorescence-labeled probe for identifying West Nile virus 2 genotype by PCR method, having direct and reverse primer activity, and a "molecular beacon" type probe which provides fluorescence detection of the amplification product with size of 116 base pairs in real time.
EFFECT: invention enables high-specific detection of said virus and can be used in epidemiological surveillance of West Nile fever in a short period of time.
1 cl, 1 dwg, 1 tbl, 3 ex
Authors
Dates
2020-03-02—Published
2019-05-21—Filed