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2 715 625

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(51)

IPC

C12Q1/68(2006-01-01)

G01N33/58(2006-01-01)

(21) (22)

Application

2019115607, 2019-05-21

(24)

Start date

2019-05-21

(22)

Actual filing date

2019-05-21

(45)

Published

2020-03-02

(72)

Inventor

Baturin Artem AleksandrovichTkachenko Galina AleksandrovnaShpak Ivan Mikhajlovich

(73)

Holder

Federalnoe Kazennoe Uchrezhdenie Zdravookhraneniya Nauchno-Issledovatelskij Protivochumnyj Institut" Federalnoj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchiya Cheloveka

SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFYING A WEST NILE VIRUS 1 GENOTYPE Russian patent published in 2020 - IPC C12Q1/68 G01N33/58

Abstract RU 2715625 C1

FIELD: biotechnology.

SUBSTANCE: invention refers to biotechnology, namely to molecular biology. A set of oligonucleotide primers and a fluorescence-labeled probe for identifying West Nile virus 1 genotype by polymerase chain reaction with reverse transcription and hybridization-fluorescence counting of results, complementary to the fragment of the West Nile polyprotein gene genotype 1, having direct and reverse primer activity, and a "molecular beacon" type probe which provides fluorescent detection of the amplification product with size of 152 base pairs in real time.

EFFECT: invention enables high-specific detection of West Nile virus of genotype 1 in biological material and can be used in epidemiological surveillance of West Nile fever.

1 cl, 1 dwg, 1 tbl, 3 ex

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RU 2 715 625 C1

Authors

Baturin Artem Aleksandrovich

Tkachenko Galina Aleksandrovna

Shpak Ivan Mikhajlovich

Dates

2020-03-02—Published

2019-05-21—Filed