FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology, namely to molecular biology. A set of oligonucleotide primers and a fluorescence-labeled probe for identifying West Nile virus 1 genotype by polymerase chain reaction with reverse transcription and hybridization-fluorescence counting of results, complementary to the fragment of the West Nile polyprotein gene genotype 1, having direct and reverse primer activity, and a "molecular beacon" type probe which provides fluorescent detection of the amplification product with size of 152 base pairs in real time.
EFFECT: invention enables high-specific detection of West Nile virus of genotype 1 in biological material and can be used in epidemiological surveillance of West Nile fever.
1 cl, 1 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
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| SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFYING A WEST NILE VIRUS GENOTYPE 2 | 2019 |
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|
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|
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| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR IDENTIFICATION OF TICK-BORNE ENCEPHALITIS VIRUS, WEST NILE FEVER, BORRELIA AND RICKETTSIA BY REAL TIME MULTIPLEX PCR | 2016 |
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| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY MARKED PROBE IN TAQMAN FORMAT FOR DETECTION OF VIRUSES OF FLAVIVIRUS GENUS BY REAL-TIME PCR | 2022 |
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| METHOD FOR DETECTING AND GENOTYPING RNA OF PORCINE REPRODUCTIVE-RESPIRATORY SYNDROME VIRUS | 2018 |
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| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBE FOR JMTV RNA IDENTIFICATION BY PCR WITH HYBRIDISATION-FLUORESCENCE DETECTION | 2023 |
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| METHOD FOR DETECTING GENOME OF ROTAVIRUS INFECTION AGENT IN FARM ANIMALS | 2018 |
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