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2 528 063

(13)

(51)

IPC

C12Q1/68(2006-01-01)

C07H21/04(2006-01-01)

(21) (22)

Application

2013106976/10, 2013-02-18

(24)

Start date

2013-02-18

(22)

Actual filing date

2013-02-18

(45)

Published

2014-09-10

(72)

Inventor

Kutsev Maksim Gennad'EvichUvarova Ol'Ga Vasil'EvnaSinitsina Tat'Jana Aleksandrovna

(73)

Holder

Federal'Noe Gosudarstvennoe Bjudzhetnoe Obrazovatel'Noe Uchrezhdenie Vysshego Professional'Nogo Obrazovanija Gosudarstvennyj Universitet"

SET OF SYNTHETIC OLIGONUCLEOTIDES FOR AMPLIFICATION AND SEQUENCING ITS1-5,8S-ITS2 OF VASCULAR PLANTS Russian patent published in 2014 - IPC C12Q1/68 C07H21/04

Abstract RU 2528063 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to a set of synthetic oligonucleotides for amplification and subsequent sequencing ITS1-5.8S-ITS2 of vascular plants, comprising carrying out a polymerase chain reaction using universal primers with the following nucleotide composition: primer ITS-for CGTAACAAGGTTTCCGTAG, primer ITS-rew GGAATCCTTGTAAGTTTCTTT.

EFFECT: invention enables to analyse effectively the structure of the internal transcribed spacer of ribosomal DNA of vascular plants.

1 dwg

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RU 2 528 063 C1

Authors

Kutsev Maksim Gennad'Evich

Uvarova Ol'Ga Vasil'Evna

Sinitsina Tat'Jana Aleksandrovna

Dates

2014-09-10—Published

2013-02-18—Filed