FIELD: biotechnology.
SUBSTANCE: method is based on single nucleotide polymorphism of genes of toxin-anatoxin systems of type II of superfamilies VapBC, HigAB and MazEF and is characterised in that for identification amplification is carried out with genomic DNA using the set of oligonucleotide primers for 10 genes of toxin-anatoxin systems. PCR products are analysed in agarose gel, the size of the obtained fragment is determined by DNA marker, the target fragments are isolated from the PCR mixture or the agarose gel and they are sequenced in order to detect the respective polymorphisms.
EFFECT: invention enables to carry out fast and accurately genotyping of mycobacteria in PCR mode and can be used for identification of clinically important strains, including with multiple and wide drug resistance, and also with altered virulence in operation with patients.
1 dwg, 3 tbl, 5 ex
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