FIELD: biotechnology, genetic engineering. SUBSTANCE: polymerase chain reaction is carried out using universal primers: 5'-CTACTAGATGGTTCGATTAGTC-3' and 5'-GTCCCTGCCGTTTGTACACA-3' for analysis of internal intergene spacer and 5'-AACTATGACTCTCTTAAGGT-3' and 5'-TCCACCAACTAAGAACGGCC-3' for analysis of external intergene spacer. Invention can be used for synthesis and the following analysis of structures of internal transcribed and external nontranscribed spacers of ribosomal eucaryotic DNA of broad group of taxons. EFFECT: improved method of analysis. 2 tbl, 3 dwg
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Authors
Dates
1998-06-20—Published
1996-08-28—Filed