FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and describes a method for determining quantitative composition of a two-component or multicomponent mixture of plants. Use of a database of correction factors for plants of different types enables to quantify the presence of each of the species in question in a complex sample, which is a mixture of an arbitrary number of different plants, using high-performance sequencing techniques. Described method is applicable both to two-component samples, and is expanded to any number of components in sample by selection of one of them as reference, and calculation of correction coefficients in relation to it.
EFFECT: invention can be used for quantitative analysis of food composition of plant origin, including in order to control such products for compliance with declared composition declared by manufacturer.
1 cl, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
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| Method for obtaining molecular single nucleotide markers for identifying an unknown individual by multiplex amplification for working with samples of degraded DNA, a kit for obtaining molecular markers | 2021 |
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|
RU2799797C2 |
| METHOD FOR PRODUCTION OF MOLECULAR MARKERS FOR DETERMINATION OF PROBABLE HUMAN APPEARANCE BY MULTIPLEX AMPLIFICATION FOR WORK WITH DEGRADED DNA SAMPLES | 2021 |
|
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| METHOD FOR DETECTING GBSSI GENE EDITING EVENTS IN CEREALS USING SET OF OLIGONUCLEOTIDE SEQUENCES | 2023 |
|
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| SAMPLE PREPARATION METHOD FOR TYPING MAJOR HISTOCOMPATIBILITY COMPLEX GENES HLA-A, HLA-B, HLA-C, HLA-DPB1, HLA-DQB1, HLA-DRB1 AND OLIGONUCLEOTIDE PRIMERS FOR ITS IMPLEMENTATION | 2023 |
|
RU2829344C1 |
| HIGH THROUGH-PUT ANALYSIS OF TRANSGENE BORDERS | 2012 |
|
RU2603265C2 |
| METHOD FOR DETERMINING MARKERS OF PRESENCE OF TUMOR T-LYMPHOBLASTS | 2016 |
|
RU2674994C2 |
