FIELD: biotechnology.
SUBSTANCE: method comprises growing cells of the strain of bacteria Rhodococcus rhodochrous RNCIM AC-1898. The cells are precipitated and washed three times with phosphate-alkaline buffered solution. It is re-suspended in the same solution and the optical density of the cell suspension is adjusted to a predetermined value, and betulin is added in the dimethyl sulphoxide solution at a concentration of from 0.5 to 3.0 g/l followed by biotransformation of betulin to betulon. And the process of biotransformation takes 24-48 hours at the optical density of the cell suspension of 2.0-2.6 (dry weight of biomass of 7-10 g/l).
EFFECT: invention enables to increase the yield of betulon.
2 cl, 2 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR BIODESTRUCTION OF DEHYDROABIETIIC ACID | 2022 |
|
RU2784816C1 |
| METHOD OF PRODUCING 5α,22α-DIHYDROXY-HYPSOGENIC ACID USING RHODOCOCCUS RHODOCHROUS CELLS | 2023 |
|
RU2810501C1 |
| METHOD OF BIOTRANSFORMATION OF PLANT MONOTERPENOID (-)-ISOPULEGOL FOR OBTAINING BIOLOGICALLY ACTIVE COMPOUNDS | 2022 |
|
RU2796679C1 |
| STRAIN Micrococcus sp., INTENDED FOR REDUCTION OF CONTENT OF TOTAL NITROGEN, AMMONIUM-ION, IRON AND ALUMINIUM IN SEWAGE WATER OF TREATMENT FACILITIES OF INDUSTRIAL ENTERPRISES | 2014 |
|
RU2567010C1 |
| BIOTRANSFORMATION PHENYLMETHYL SULPHIDE IN (R)-SULPHOXIDE USING IMMOBILIZED CELLS GORDONIA TERRAE IEGM 136 | 2015 |
|
RU2607027C1 |
| STRAIN OF RHODOCOCCUS RUBER IEGM 346 - DICLOFENAC SODIUM BIODESTRUCTOR | 2018 |
|
RU2707536C1 |
| IMMOBILISED BIOCATALYST FOR MICROBIAL BIOTRANSFORMATION OF STEROID COMPOUNDS | 2013 |
|
RU2524434C1 |
| METHOD OF BIODEGRADATION OF DROTAVERINE HYDROCHLORIDE | 2012 |
|
RU2496866C1 |
| IBUPROFEN BIODESTRUCTOR | 2021 |
|
RU2762007C1 |
| STRAIN OF BACTERIA BACILLUS VALLISMORTIS - DESTRUCTOR OF OIL AND OIL PRODUCTS | 2013 |
|
RU2513702C1 |
