FIELD: biotechnology.
SUBSTANCE: immobilised biocatalyst for microbial biotransformation of steroid compounds comprises cells of a microorganism having 1,2-dehydrogenase activity, included in the polyvinyl alcohol cryogel matrix. As cell having 1,2-dehydrogenase activity the immobilised biocatalyst comprises biomass of actinobacteria Pimelobacter simplex. The ratio of components in the biocatalyst is (wt %): actinobacteria cells Pimelobacter simplex - 1-5 (in dry substance), polyvinyl alcohol - 7-20, the aqueous phase - up to 100.
EFFECT: implementation of stereo, regioselective biotransformation of respective steroid substrates at their elevated initial concentration, obtaining the desired product with high yield, the ability to perform multiple, at least 30 cycles, use of the biocatalyst without loss of activity.
1 dwg, 1 tbl, 1 ex
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