NUCLEIC ACID HAVING ACTIVITY OF SHORT INTERFERING RNA, VECTOR CONTAINING THIS NUCLEIC ACID COUPLED OPERABLY WITH SEQUENCES OF TRANSCRIPTION REGULATION, AND METHOD OF THEIR APPLICATION FOR INHIBITION OF CELL PROLIFERATION OF HUMAN PANCREATIC ADENOCARCINOMA Russian patent published in 2014 - IPC C12N15/11 C12N15/113 C12N15/63 A61K31/7105 A61P35/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to molecular biology, in particular to short interfering RNA (siRNA), and can be used in antitumor therapy. On the basis of genome analysis the sequences of siRNA against the human gene HIF1A with SEQ ID NO:1-2 are designed, siRNA against the human gene HSP8A with SEQ ID NO:3-4, siRNA against the human gene APEX1 with SEQ ID NO:5-6, and siRNA against the human gene CCND3 with SEQ ID NO:7-8, associated with cell proliferation of human pancreatic adenocarcinoma. Using the obtained siRNA, including as part of a lentiviral vector, results in suppression of cell proliferation of human pancreatic adenocarcinoma and tumor destruction. The invention enables to achieve the level of suppression of proliferation up to 65% using siRNA to genes HIF1A and HSP8A associated with stress and responsible for the major proliferative (CCND3) and reparative (APEX-1) ways of cell division.

EFFECT: improving level of suppression of proliferation.

3 cl, 10 dwg, 2 ex