METHOD OF ELECTROCHEMICAL IDENTIFICATION OF TARGET SEQUENCES OF NUCLEOTIDES Russian patent published in 2014 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: method comprises obtaining the biological sample which may contain a target nucleotide sequence. The means of amplification are provided, comprising DNA polymerase, a pair of primers and four free nucleotides. The compound is provided, capable of oxidation-reduction, which is capable of intercalation during replication between the nucleotides forming the said copied target sequences, and the said copied target sequences cause inhibition of electrochemical activity of the said intercalated compound capable of oxidation-reduction, due to which the electric current decreases. The DNA polymerase, a pair of primers and four free nucleotides are activated using temperature. The electric field is applied to the said sample to activate the compound capable of oxidation-reduction. The initial parameters of the electric current are measured. The electric current is measured which represents the electrochemical activity of the compound capable of oxidation-reduction, which passes through the said sample. The presence of the given target nucleotide sequence is determined, if the electric current decreases.

EFFECT: invention enables to identify the target nucleotide sequences with high precision.

19 cl, 11 dwg, 1 tbl