FIELD: medicine.
SUBSTANCE: device for polymerase chain reaction comprises at least four portions, each of which has two flat parallel surfaces perpendicular to a common axis, and holes. The portions are rotated in relation to each other and the common axis. The first portion has at least six through holes connecting its two flat surfaces, at least four holes of which are provided with hydrophilic filters mounted to contact the flat surface of the second portion. The second portion is configured with at least two through holes connecting its two flat surfaces, at least one hole of which comprises a sorbent fixed by the hydrophilic filters contacting the flat surfaces of the first and third portions. The third portion has at least two through holes connecting its two flat surfaces, at least one hole of which comprises the hydrophilic filter mounted to contact the flat surfaces of the second and fourth portions, and at least three blind holes, each of which comprises the hydrophilic filters mounted to contact the flat surface of the second portion. The fourth portion is provided with at least one blind optically transparent through hole, comprising the hydrophilic filter mounted to contact the flat surface of the third portion non contacting the bottom of the hole containing it.
EFFECT: enhancement involving carrying out the stages of nucleic acid recovery and PCR in the same apparatus with detecting reaction results and simplifying the device.
8 cl, 5 dwg
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