REPLACEABLE MICROFLUID MODULE FOR AUTOMATED RECOVERY AND PURIFICATION OF NUCLEIC ACIDS FROM BIOLOGICAL SAMPLES AND METHOD FOR RECOVERY AND PURIFICATION NUCLEIC ACIDS WITH USING THEREOF Russian patent published in 2010 - IPC C12N15/10 

FIELD: medicine.

SUBSTANCE: replaceable microfluid module for automated recovery and purification of nucleic acids from biological samples contains reagent reservoirs and reaction capacities, microchannels and valves required to handle the liquids in the module. The module comprises an inleak chamber for introduction of the biological sample, a lysis reservoir containing a membrane, reservoirs for dissolved lytic buffers, a microcolumn containing a solid-phase sorbent for binding nucleic acids, a reservoir for flush buffer and ethanol to discharge residual proteins and salt crystals from the microcolumn, an eluting buffer reservoir, an outlet port compatible to a standard microtube, a waste collection reservoir. The method for recovery and purification of nucleic acids from biological samples involves filtration of a sample through the membrane, lysis of the microorganism cells kept on the membrane, binding of nucleic acids on the microcolumn, washing nucleic acids from proteins and salt crystals, elution of nucleic acids from the microcolumn in the tube. All the stages of recovery and purification are carried out in automatic mode in the microfluid module that isolates the sample from environment.

EFFECT: invention allows reducing cost price of the nucleic acid preparation.

14 cl, 17 dwg, 1 tbl, 8 ex