DOSAGE FORM FOR AMPLIFICATION OF NUCLEIC ACIDS Russian patent published in 2020 - IPC C12Q1/68 

FIELD: medicine; biology.

SUBSTANCE: invention relates to medicine and biology and is intended for use in production of sets for amplification of nucleic acids, including in production of sets for etiological diagnosis of COVID-19. Dosage form for amplification of nucleic acids includes a glass-like non-porous core containing ficoll, galactomannan and an aqueous solution of the following components: primers, a thermostable enzyme of DNA polymerase and a mixture of four deoxynucleoside triphosphates, as well as a continuous shell consisting of mineral oil, wherein content of ficoll, galactomannan and aqueous solution in core, wt%: ficoll – 10.0–30.0, galactomannan – 0.1–5.0, and aqueous solution – the rest. Dosage form can be presented in form of globule or tablet, wherein globule has preferred diameter of 0.5–4.0 mm, and tablet has preferred diameter of 0.5–4.0 mm and preferential maximum thickness of 0.5–4.0 mm. Aqueous solution can include as additional components any labeled probes for nucleic acid amplification reaction and reverse transcriptase.

EFFECT: invention allows to prevent uncontrolled abrasion of the dosage form for amplification of nucleic acids.

4 cl, 16 ex