DRY MIXTURE FOR PREPARING REACTION MIXTURE FOR NUCLEIC ACID AMPLIFICATION AND METHOD FOR PRODUCTION THEREOF Russian patent published in 2014 - IPC C12Q1/68 

Abstract RU 2535995 C2

FIELD: chemistry.

SUBSTANCE: invention relates to molecular biology and biotechnology and a dry mixture for nucleic acid amplification and a method for production thereof. The described mixture contains a thermally stable DNA polymerase, a reverse transcriptase, a first stabiliser selected from a group including monosaccharides, disaccharides, polyalcohols and a second stabiliser selected from a group including polysaccharides, albumins and polyvinylpyrrolidone. The method of producing the dry mixture includes preparation of an aqueous solution containing said substances and lyophilisation thereof. The obtained product can be stored for up to 1 year at temperature higher than 0°C while preserving reverse transcriptase and DNA polymerase activity.

EFFECT: invention can be used for amplification of DNA and RNA simultaneously and separately.

26 cl, 13 dwg, 7 tbl, 4 ex

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RU 2 535 995 C2

Authors

Petrov Vadim Viktorovich

Kuevda Dmitrij Aleksandrovich

Shipulin German Aleksandrovich

Dates

2014-12-20Published

2012-06-01Filed