FIELD: chemistry.
SUBSTANCE: group of inventions including a composition for a hot start reverse transcription reaction, a method for preparing the above composition, a reverse transcription and hot start PCR composition, a method for preparing the above composition, a hot start reverse transcription reaction kit that includes a reaction composition of reverse transcription with a hot start and a reaction tube, a set for reverse transcription and hot start PCR, a reverse transcription method for the RNA template, an amplification method of nucleic acid, a PCR composition with reverse transcription for the diagnosis of malignant neoplasm. The composition for the reverse transcription reaction contains in its composition the Mg2+, four types of dNTP, reverse transcriptase, pyrophosphate and pyrophosphatase. The PCR composition, in addition to the above, includes a DNA polymerase.
EFFECT: invention expands the arsenal of means allowing to carry out the reverse transcription reaction.
37 cl, 13 dwg, 3 tbl, 13 ex
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Authors
Dates
2017-09-15—Published
2013-03-11—Filed