COMPOSITIONS FOR THE REACTION OF REVERSE TRANSCRIPTION WITH A HOT START OR FOR A POLYMERASE CHAIN REACTION WITH REVERSE TRANSCRIPTION WITH A HOT START Russian patent published in 2017 - IPC C12Q1/42 C12Q1/48 C12Q1/68 

FIELD: chemistry.

SUBSTANCE: group of inventions including a composition for a hot start reverse transcription reaction, a method for preparing the above composition, a reverse transcription and hot start PCR composition, a method for preparing the above composition, a hot start reverse transcription reaction kit that includes a reaction composition of reverse transcription with a hot start and a reaction tube, a set for reverse transcription and hot start PCR, a reverse transcription method for the RNA template, an amplification method of nucleic acid, a PCR composition with reverse transcription for the diagnosis of malignant neoplasm. The composition for the reverse transcription reaction contains in its composition the Mg²⁺, four types of dNTP, reverse transcriptase, pyrophosphate and pyrophosphatase. The PCR composition, in addition to the above, includes a DNA polymerase.

EFFECT: invention expands the arsenal of means allowing to carry out the reverse transcription reaction.

37 cl, 13 dwg, 3 tbl, 13 ex