METHODS AND REAGENT KITS FOR GENE-AMPLIFICATION DIAGNOSTICS INVOLVING PREPARATION AND RNA PREPARATION METHODS Russian patent published in 2001 - IPC

FIELD: transfusion techniques and epidemiology. SUBSTANCE: biological material is centrifuged to precipitate ballast substances and then cellular and viral precipitate of biological material is lysed. Viral, bacterial, and cellular DNA and RNA are precipitated by isopropyl alcohol and transferred from organic medium into dry precipitate, which is treated by reverse transcriptase in buffer, containing triple-substituted phosphates and primers for RNA, to give summary DNA and cDNA preparation for precipitation assays avoiding isolation of RNA. Precipitation assay is carried out by mixing DNA preparation with preliminarily prepared reagents: double buffer with enzyme Taq polymerase and gene amplification reagent specific for each infection agent and analyzed gene, which contains primers and triple-substituted phosphates. Reagent kits are used for diagnosing hepatitis B and C, HIV infections and lues enabling detection of several infections in the same sample. EFFECT: facilitated infection diagnostics. 12 cl, 8 dwg, 7 ex