METHOD AND APPARATUS FOR ANTIGEN RETRIEVAL PROCESS Russian patent published in 2015 - IPC G01N1/30 G01N33/543 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to a method for retrieval of an antigen in a formaldehyde-fixed tissue sample, and to a kit used in said method. The method includes incubating the formaldehyde-fixed tissue sample in a first antigen retrieval solution at a temperature higher than 90°C; transferring the formaldehyde-fixed tissue sample to a second antigen retrieval solution; and incubating the formaldehyde-fixed tissue sample in the second antigen retrieval solution at a temperature higher than 90°C. The first antigen retrieval solution comprises a buffer solution having a pH range of between about 5 and about 7 and the second antigen retrieval solution comprises a buffer solution having a pH range of between about 7.5 and about 11. Alternatively, the first antigen retrieval solution comprises a buffer solution having a pH range of between about 7.5 and about 11 and the second antigen retrieval solution comprises a buffer solution having a pH range of between about 5 and about 7. The kit comprises a first antigen retrieval solution which retrieves at least a portion of unretrieved antigens in the sample, and a second antigen retrieval solution which retrieves at least some of another portion of unretrieved antigens in the sample. The first solution comprises citric acid, potassium dihydrogen phosphate, boric acid, diethyl barbituric acid, piperazine-N,N′-bis(2-ethanesulphonic acid), dimethylarsinic acid, 2-(N-morpholino)ethanesulphonic acid, or a combination thereof, and the second solution comprises tris(hydroxymethyl)methylamine (TRIS), 2-(N-morpholino)ethanesulphonic acid (TAPS), N,N′-bis(2-hydroxyethyl)glycine (Bicine), N-tris(hydroxymethyl)methylglycine (Tricine), 4-2-hydroxyethyl-1-piperazineethanesulphonic acid (HEPES), 2-{[tris(hydroxymethyl)methyl]amino}ethanesulphonic acid (TES), or a combination thereof.

EFFECT: said steps of using the first and second antigen retrieval solutions improve antigen retrieval in tissue compared to use of the first solution without the second solution and vice versa.

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