GENETIC MAKER TO ENSURE EXPRESSION OF TARGET HOMOLOGOUS AND HETEROLOGOUS GENES IN CELLS OF FILAMENTOUS FUNGUS PENICILLIUM VERRUCULOSUM; USED AS HOST, METHOD FOR MAKING STRAIN OF FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR MAKING ENZYMATIC AGENT Russian patent published in 2010 - IPC C12N15/00 C12N9/24 C12N1/15 C12R1/80 

Abstract RU 2378372 C2

FIELD: medicine.

SUBSTANCE: invention represents the method for making an enzyme, preferentially cellulase or xynalase, decomposing cellulose or xylenes respectively by cultivation of strain of fungus Penicillium verruculosum. Said strain is derived from transformation of a cell of fungus Penicillium verruculosum with a genetic maker containing a target coding enzyme sequence functionally connected with regulatory gene elements of cellobiohydrolase I Penicillium verruculosum which represent a promotor and terminator of a cellobiohydrolase I gene, while an identification peptide herewith depends on the fact what target enzyme is.

EFFECT: invention allows producing high efficient enzymes.

6 cl, 6 dwg, 1 tbl, 4 ex

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RU 2 378 372 C2

Authors

Sinitsyn Arkadij Pantelejmonovich

Rozhkova Aleksandra Mikhajlovna

Sinitsyna Ol'Ga Arkad'Evna

Fedorova Ekaterina Aleksandrovna

Okunev Oleg Nikolaevich

Bekkarevich Aleksandra Olegovna

Sokolova Lidija Mikhajlovna

Matys Veronika Jur'Evna

Koshelev Anatolij Vladimirovich

Vinetskij Jurij Pavlovich

Chernoglazov Vladimir Mikhajlovich

Zorov Ivan Nikitovich

Dates

2010-01-10Published

2008-03-03Filed