FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. Recombinant strain of the filamentous fungus Penicillium canescens CL14 ECM F-4706D, producing bacterial thermophilic cellulase Cel5L from Clostridium thermocellum with a molecular weight of 48 kDa is proposed. This strain is obtained by transforming the original P. canescens RN3-11-7 strain niaD(-) with a plasmid carrying the celL gene encoding Cel5L cellulase from the Clostridium thermocellum bacterium. Method for cultivating this strain is also proposed, comprising growing the strain on a nutrient medium of the following composition (g/l): soybean meal – 45, corn extract – 40, KH2PO4 – 6, CaCl2 – 0,23, MgSO4×7H2O – 0,3, yeast extract – 10, wheat bran – 10.
EFFECT: group of inventions provides an effective production of cellulase Cel5L from Clostridium thermocellum in a heterologous fungal host strain.
2 cl, 6 dwg, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NEW RECOMBINANT STRAIN OF FILAMENTOUS FUNGI PENICILLIUM CANESCENS CS15, PRODUCING CELLULASE CLOSTRIDIUM THERMOCELLUM AND METHOD OF ITS CULTIVATION | 2015 |
|
RU2612158C1 |
| NEW RECOMBINANT MYCELIAL FUNGUS PENICILLIUM CANESCENS PEP3 STRAIN AND PRODUCING A COMPLEX ENDO- AND EXO-PROTEOLYTIC ENZYME PREPARATION BASED ON THEREOF | 2015 |
|
RU2616275C1 |
| NEW RECOMBINANT STRAIN (VERSIONS) OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM, AND ENZYMATIC AGENT (VERSIONS) INTENDED FOR HYDROLYSIS OF FRUIT RAW MATERIAL, AND METHOD OF ITS PRODUCTION | 2014 |
|
RU2574206C1 |
| PENICILLIUM CANESCENS MTCBHI FUNGUS STRAIN PRODUCER OF THERMALLY STABLE CELLOBIOHYDROLASE I AND AN ENZYME PREPARATION BASED THEREON FOR BIOCONVERSION OF RENEWABLE VEGETAL RAW MATERIAL INTO SUGARS | 2019 |
|
RU2741078C1 |
| PENICILLIUM VERRUCULOSUM MX-73 FUNGUS STRAIN PRODUCER OF MODIFIED XYLANASE E WITH HIGH HEAT STABILITY, ENZYME PREPARATION BASED THEREON FOR USE IN FOOD AND FEED INDUSTRY AND METHOD FOR PRODUCTION THEREOF | 2018 |
|
RU2711578C1 |
| STRAIN OF PENICILLIUM VERRUCULOSUM PRODUCER OF PHYTASE AND ENDO-1,4-β-GLUCANASE II COMPLEX AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2022 |
|
RU2810538C2 |
| METHOD OF PRODUCING ENZYMATIC AGENT FOR DEGRADATION OF HEMICELLULOSE HETEROPOLYSACCHARIDES OF HERBAL CYTODERM AND ENZYMATIC AGENT (VERSIONS) | 2007 |
|
RU2358756C1 |
| GENETIC MAKER TO ENSURE EXPRESSION OF TARGET HOMOLOGOUS AND HETEROLOGOUS GENES IN CELLS OF FILAMENTOUS FUNGUS PENICILLIUM VERRUCULOSUM; USED AS HOST, METHOD FOR MAKING STRAIN OF FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR MAKING ENZYMATIC AGENT | 2008 |
|
RU2378372C2 |
| PENICILLIUM VERRUCULOSUM FUNGUS STRAIN AS PRODUCER OF COMPLEX OF PHYTASE A AND ENDO-1,4-β-XYLANASE E AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2023 |
|
RU2819918C1 |
| GENETIC CONSTRUCT FOR EXPRESSION OF A COMPLEX OF ENZYMES OF ENDOGLUCANASES AND XYLANASES IN THE CELLS OF THE FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR OBTAINING COMPLEX ENZYME PREPARATIONS BASED ON IT, INTENDED FOR FODDER PRODUCTION | 2017 |
|
RU2653429C1 |
