FIELD: biotechnology.
SUBSTANCE: invention relates to a recombinant strain of filamentous fungi Penicillium canescens CS15 VKM F-4679D, secreting thermostable cellulase Cel48S from Clostridium thermocellum. The invention also discloses a method for culturing of the said strain in a particular nutrient medium. The said nutrient medium consists of 35 g/l of soybean hulls, 40 g/l of corn extract, 6 g/l of KH2PO4, 0.23 g/l of CaCl2, 0.3 g/l of MgSO4* 7H2O, 10 g/l of yeast extract and 10 g/l of wheat bran.
EFFECT: invention allows efficient production of cellulase from Clostridium thermocellum in a heterologous host Penicillium canescens.
2 cl, 6 dwg, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT STRAIN OF MITSENAL MUSHROOM PENICILLIUM CANESCENS CL14, PRODUCTION COMPONENT OF CELLULOSUM CLOSTRIDIUM THERMOCELLUM AND METHOD OF ITS CULTIVATION | 2016 |
|
RU2646132C1 |
| RECOMBINANT STRAIN OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM (VERSIONS) AND A METHOD OF PRODUCING ENZYME PREPARATION WITH ITS USE (VARIANTS) | 2015 |
|
RU2646136C2 |
| NEW RECOMBINANT STRAIN (VERSIONS) OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM, AND ENZYMATIC AGENT (VERSIONS) INTENDED FOR HYDROLYSIS OF FRUIT RAW MATERIAL, AND METHOD OF ITS PRODUCTION | 2014 |
|
RU2574206C1 |
| FUNGAL STRAIN Penicillium verruculosum B10 EGII PRODUCER OF ENDO-1,3/1,4-β-GLUCANASE, CELLULASE, β-GLUCOSIDASE AND XYLANASE AND METHOD OF PRODUCTION OF FODDER COMPLEX ENZYME PREPARATION | 2012 |
|
RU2532840C2 |
| DNA FRAGMENT OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM ENCODING SYNTHESIS OF SECRETABLE ENDOGLUCANASE III AND GROUP OF PENICILLIUM CANESCENS STRAINS PRODUCING PENICILLIUM VERRUCULOSUM ENDOGLUCANASE III CONSTRUCTED BY METHODS OF TRANSFORMATION AND GENETIC ENGINEERING BASED ON THIS DNA FRAGMENT | 2001 |
|
RU2238974C2 |
| GENETIC CONSTRUCT FOR EXPRESSION OF A COMPLEX OF ENZYMES OF ENDOGLUCANASES AND XYLANASES IN THE CELLS OF THE FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR OBTAINING COMPLEX ENZYME PREPARATIONS BASED ON IT, INTENDED FOR FODDER PRODUCTION | 2017 |
|
RU2653429C1 |
| CELL OF MYCELIAL FUNGUS Penicillium canescens - XYLANASE AND LACCASE PRODUCENT, METHOD OF OBTAINING COMBINED EXYME XYLANASE AND LACCASE PREPARATION | 2012 |
|
RU2538149C2 |
| PENICILLIUM CANESCENS MTCBHI FUNGUS STRAIN PRODUCER OF THERMALLY STABLE CELLOBIOHYDROLASE I AND AN ENZYME PREPARATION BASED THEREON FOR BIOCONVERSION OF RENEWABLE VEGETAL RAW MATERIAL INTO SUGARS | 2019 |
|
RU2741078C1 |
| GENETIC MAKER TO ENSURE EXPRESSION OF TARGET HOMOLOGOUS AND HETEROLOGOUS GENES IN CELLS OF FILAMENTOUS FUNGUS PENICILLIUM VERRUCULOSUM; USED AS HOST, METHOD FOR MAKING STRAIN OF FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR MAKING ENZYMATIC AGENT | 2008 |
|
RU2378372C2 |
| METHOD OF PRODUCING ENZYMATIC AGENT FOR DEGRADATION OF HEMICELLULOSE HETEROPOLYSACCHARIDES OF HERBAL CYTODERM AND ENZYMATIC AGENT (VERSIONS) | 2007 |
|
RU2358756C1 |
