FIELD: biotechnology.
SUBSTANCE: said plasmid is characterized by presence of the following fragments: a NcoI/SalI fragment of pET-40b(+) plasmid (Novagen) and a 2577 bp DNA fragment containing a chimeric gene encoding the full-length alkaline phosphatase CmAP and the full-length Yersinia pseudotuberculosis OmpF porin, linked together by a sequence encoding a flexible linker (G4S)3 (SEQ ID NO: 1). A recombinant strain of E. coli Rosetta (DE3)/pET40CmAP/OmpF, which is a producer of a bifunctional hybrid CmAP/OmpF polypeptide, and which is obtained by modification of the E. coli Rosetta (DE3) strain with this pET40CmAP/OmpF plasmid, is proposed.
EFFECT: high yield of hybrid protein with the properties of recombinant highly-active alkaline phosphatase and pore-forming protein.
2 cl, 2 dwg, 3 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA pET40CmAP/CGL ENCODING HYBRID BIFUNCTIONAL POLYPEPTIDE CmAP/CGL WITH PROPERTIES OF HIGHLY ACTIVE ALKALINE PHOSPHATASE CmAP AND GALACTOSE SPECIFIC LECTIN CGL, RECOMBINANT STRAIN OF E coli Rosetta (DE3)/pET40CmAP/CGL - PRODUCER OF HYBRID BIFUNCTIONAL POLYPEPTIDE CmAP/CGL AND METHOD OF ITS PREPARATION |
2014 |
- Balabanova Larisa Anatol'Evna
- Golotin Vasilij Aleksandrovich
- Koval'Chuk Svetlana Nikolaevna
- Chernikov Oleg Viktorovich
- Chikalovets Irina Vladimirovna
- Rasskazov Valerij Aleksandrovich
|
RU2557306C1 |
| RECOMBINANT PLASMID DNA pET40CmAP/MBL-T CODING HYBRID BIFUNCTIONAL POLYPEPTIDE CmAP/MBL-T WITH PROPERTIES OF HIGH-ACTIVITY ALKALINE PHOSPHATASE CmAP AND MANNAN-BINDING LECTIN OF C-TYPE MBL-T, RECOMBINANT STRAIN E coli Rosetta(DE3)/pET40CmAP/MBL-T - PRODUCER OF HYBRID BIFUNCTIONAL POLYPEPTIDE CmAP/MBL-T, AND METHOD FOR ITS OBTAINING |
2013 |
- Balabanova Larisa Anatol'Evna
- Koval'Chuk Svetlana Nikolaevna
- Golotin Vasilij Aleksandrovich
- Bulgakov Aleksandr Aleksandrovich
- Rasskazov Valerij Aleksandrovich
|
RU2538169C1 |
| ALKALINE PHOSPHATASE CmAP SYNTHESIS-DETERMINING 40Ph PLASMID, E. coli rosetta(DE3)/40Ph STRAIN - PRODUCER OF CHIMERIC PROTEIN, CONTAINING AMINO ACID SEQUENCE OF RECOMBINANT ALKALINE PHOSPHATASE CmAP, AND PRODUCTION METHOD THEREOF |
2010 |
- Balabanova Larisa Anatol'Evna
- Rasskazov Valerij Aleksandrovich
|
RU2447151C1 |
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2015 |
- Petrovskaya Lada Evgenevna
- Shingarova Lyudmila Nikolaevna
- Kryukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Zlobinov Aleksandr Vladimirovich
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- Kirpichnikov Mikhail Petrovich
|
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2015 |
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|
RU2603092C1 |
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2022 |
- Gerasimov Andrei Sergeevich
- Misterova Anna-Anastasiia Viktorovna
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|
RU2799530C1 |
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2014 |
- Frank Ljudmila Alekseevna
- Tikunova Nina Viktorovna
- Burakova Ljudmila Petrovna
- Bajkov Ivan Konstantinovich
- Kudrjavtsev Aleksandr Nikolaevich
- Morozova Vera Vital'Evna
|
RU2565545C1 |
| RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli |
2013 |
- Petrovskaja Lada Evgen'Evna
- Shingarova Ljudmila Nikolaevna
- Gapizov Sultan Shakhbanovich
- Krjukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Jakimov Sergej Aleksandrovich
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|
RU2527171C1 |
| RECOMBINANT PLASMID DNA pET19b-SurvOL, WHICH PROVIDES SYNTHESIS OF A HYBRID PROTEIN SURVIVIN-OBELIN (SurvOL) AND A HYBRID PROTEIN BOUND BY ANTI-SURVIVIN ANTIBODIES AND POSSESSING BIOLUMINESCENT ACTIVITY |
2021 |
- Bashmakova Evgeniia Evgenevna
- Panamarev Nikita Sergeevich
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|
RU2770490C1 |
| RECOMBINANT PLASMID DNA pEst877 DETERMINING EXPRESSION OF POLYPEPTIDE WITH ACTIVITY OF ESTERASE Psychrobacter cryohalolentis K5 ON CELL SURFACE Escherichia coli, AND BACTERIA STRAIN Escherichia coli BL21 (DE3) pLysS/pEst877-PRODUCER OF POLYPEPTIDE WITH ACTIVITY OF ESTERASE PSYCHROBACTER CRYOHALOLENTIS K5 ON CELL SURFACE |
2013 |
- Petrovskaja Lada Evgen'Evna
- Krjukova Elena Aleksandrovna
- Novototskaja-Vlasova Ksenija Aleksandrovna
- Rivkina Elizaveta Mikhajlovna
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|
RU2526213C1 |
