FIELD: biotechnology.
SUBSTANCE: said plasmid is characterized by presence of the following fragments: a NcoI/SalI fragment of pET-40b(+) plasmid (Novagen) and a 2577 bp DNA fragment containing a chimeric gene encoding the full-length alkaline phosphatase CmAP and the full-length Yersinia pseudotuberculosis OmpF porin, linked together by a sequence encoding a flexible linker (G4S)3 (SEQ ID NO: 1). A recombinant strain of E. coli Rosetta (DE3)/pET40CmAP/OmpF, which is a producer of a bifunctional hybrid CmAP/OmpF polypeptide, and which is obtained by modification of the E. coli Rosetta (DE3) strain with this pET40CmAP/OmpF plasmid, is proposed.
EFFECT: high yield of hybrid protein with the properties of recombinant highly-active alkaline phosphatase and pore-forming protein.
2 cl, 2 dwg, 3 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA pET40CmAP/CGL ENCODING HYBRID BIFUNCTIONAL POLYPEPTIDE CmAP/CGL WITH PROPERTIES OF HIGHLY ACTIVE ALKALINE PHOSPHATASE CmAP AND GALACTOSE SPECIFIC LECTIN CGL, RECOMBINANT STRAIN OF E coli Rosetta (DE3)/pET40CmAP/CGL - PRODUCER OF HYBRID BIFUNCTIONAL POLYPEPTIDE CmAP/CGL AND METHOD OF ITS PREPARATION |
2014 |
- Balabanova Larisa Anatol'Evna
- Golotin Vasilij Aleksandrovich
- Koval'Chuk Svetlana Nikolaevna
- Chernikov Oleg Viktorovich
- Chikalovets Irina Vladimirovna
- Rasskazov Valerij Aleksandrovich
|
RU2557306C1 |
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2013 |
- Balabanova Larisa Anatol'Evna
- Koval'Chuk Svetlana Nikolaevna
- Golotin Vasilij Aleksandrovich
- Bulgakov Aleksandr Aleksandrovich
- Rasskazov Valerij Aleksandrovich
|
RU2538169C1 |
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2010 |
- Balabanova Larisa Anatol'Evna
- Rasskazov Valerij Aleksandrovich
|
RU2447151C1 |
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2015 |
- Petrovskaya Lada Evgenevna
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- Kryukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Zlobinov Aleksandr Vladimirovich
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- Kirpichnikov Mikhail Petrovich
|
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2015 |
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|
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2022 |
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|
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2014 |
- Frank Ljudmila Alekseevna
- Tikunova Nina Viktorovna
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- Bajkov Ivan Konstantinovich
- Kudrjavtsev Aleksandr Nikolaevich
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|
RU2565545C1 |
| RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli |
2013 |
- Petrovskaja Lada Evgen'Evna
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- Gapizov Sultan Shakhbanovich
- Krjukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
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|
RU2527171C1 |
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2021 |
- Bashmakova Evgeniia Evgenevna
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|
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2012 |
- Balabanova Larisa Anatol'Evna
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|
RU2504583C1 |
