FIELD: biotechnology, genetic engineering.
SUBSTANCE: following is proposed: pCSBrz4M recombinant plasmid encoding the full amino acid sequence of the hybrid polypeptide with the properties of the sweet protein brazzein providing stable expression of the hybrid polypeptide, as well as a high level of accumulation of the target protein in a soluble form in the cytoplasm of E. coli. A recombinant strain of E. coli_BL21_pCSBrz4M is also proposed, which is a producer of a hybrid polypeptide with the properties of brazzein sweet protein, and obtained by transforming cells of the E. coli BL21 strain with pCSBrz4M recombinant plasmid vector.
EFFECT: inventions make it possible to carry out a highly efficient synthesis of a hybrid polypeptide with the properties of brazzein sweet protein.
2 cl, 3 dwg, 3 ex
| Title |
Year |
Author |
Number |
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