METHOD OF OBTAINING MAGNETIC AFFINITY SORBENT FOR SEPARATION OF RECOMBINANT PROTEINS Russian patent published in 2015 - IPC B01J20/30 B01J20/281 B01D15/38 

FIELD: chemistry.

SUBSTANCE: claimed is method of obtaining magnetic affinity sorbent for separation of recombinant proteins. Method includes application of porous silica layer on the surface of magnetic microspheres of volatile ashes from coal combustion and impregnation with solution, containing ions of transition metals. As microspheres fraction of magnetic microspheres, containing 40-41 wt % of glass phase, is used. Application of porous silica layer is realised in hydrothermal conditions. Magnetic microspheres contact with liquid reaction mixture, containing tetraethoxysilane and hexadecyltriammonium bromide as structure-forming agent. After application of silica layer solid phase is separated, washed, thermally processed and activated by boiling in water medium.

EFFECT: invention makes it possible to increase strength of silica envelope on the surface of microspheres without reduction of sorption capacity by medium-size proteins.

7 cl, 2 dwg, 3 tbl, 9 ex