METHOD FOR CULTURING ONECELLULAR GREEN ALGAE HAEMATOCOCCUS PLUVIALIS FOR ASTAXANTHIN PRODUCTION Russian patent published in 2015 - IPC C12N1/12 

FIELD: biotechnology.

SUBSTANCE: invention refers to a method for culturing unicellular green algae Haematococcus pluvialis for astaxanthin production involving inducing astaxanthin synthesis in monade vegetative cells with the culture grown on the nutrient medium MONM-1 in substrate cell saturation with biogenic elements (inoculate) is introduced in an amount of 0.3-0.3510⁷cell.l^-1 into the nutrient medium MONM-2 different from the medium MONM-1 by 30-fold reduced content of nitrogen (0.2 mM.l^-1) and phosphorus (0.12 mm.l^-1); sodium acetate 15 mM is once introduced that is followed by 20-day semi-flow growth (0.1-0.3 day"1) with maintaining the target levels of nitrogen and phosphorus and exposing to luminescent day-light lamp at a light intensity of 120 mcE.g^-2·s^-1, with continuous air blow (0.3 l min"1) and temperature 22-26 °C. Developed is a cost-effective and efficient method, which can provide a basis for industrial culture of the unicellular green algae Haematococcus pluvialis as raw material for producing pure natural astaxanthin and a biologically active additive with high carotinoid bioavailability.

EFFECT: method prevents the vegetative cell transformation into aplanospores, provides the advantage in the monade cell cultures (>80%) over at least 20 days and the astaxanthin content in the biomass of at least 2% of dry matter.