BACTERIA Bacillus subtilis PRODUCING 5`-AMINOIMIDAZOLE-4-CARBOXAMIDERIBOSIDE (AICAR), AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF AICAR BY CULTURING THIS BACTERIUM Russian patent published in 2015 - IPC C12N1/21 C12P19/40 C12R1/125 

FIELD: biotechnology.

SUBSTANCE: bacterium Bacillus subtilis is proposed, producing 5′-aminoimidazole-4-carboxamideriboside (AICAR). The said bacterium comprises deregulated pur-operon against the background of inactivated gene purH, the modified heterologous genes prs and purF E.coli under the control of a strong promoter P_rpsF as part of chromosome. At that it has one of the following characteristics: comprises the gene zwf under control of a strong promoter P_rpsF, comprises the heterologous gene udhA under the control of a strong promoter P_rpsF, comprises the deletion of the gene sacB. The method of synthesizing AICAR by culturing the said bacteria under proper conditions is also proposed. At that culturing is carried out on the medium of the following composition, wt %: fodder yeast 0.5-1.0, sucrose 10-13, soy isolate 2.5-3.5, corn-steep extract 3.0-5.0, urea 0,6-0,8, (H3₄)₂3CO₄ 0.8-1.6, propinol 0.4-0.5, water - the rest.

EFFECT: high level of synthesis of AICAR.

5 cl, 2 tbl, 9 ex