FIELD: biotechnologies.
SUBSTANCE: invention proposes a method for quantitative determination of species composition of propionibacteria dwelling on human skin. The method provides for acquisition of a sample, extraction of RNA from it and performance of a quantitative PCR reaction. The reaction is performed with primers GCG TGA GTG ACG GTA ATG GGT A, TTC CGA CGC GAT CAA CCA, FAM-AGC GTT GTC CGG ATT TAT TGG GCG-BHQ1, ACA TGG ATC CGG GAG CTT C, ACC CAA CAT CTC ACG ACA CG, R6G-CGG TTC ACA GGT GGT GCA TTG GC-BHQ2, GTC TGC AAC TCG ACC CCA T, AGT CCT AAT TAC CAG TCC CAC, ROX-ACC TGT GTG GGG GAG CCG TCG AAG-BHQ2, AGA ACT CTG AGC GCT AGC TGT AG, CGT AGA ACT AGC TGT AGC GCA, Cy5-AG CGG CTC CTA CTT CTG CAG GGG-BHQ2.
EFFECT: method allows reducing time of investigations and performing a quantitative estimation of species composition of propionibacteria dwelling on human skin.
2 tbl
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