FIELD: biotechnology.
SUBSTANCE: method of production of peptides is proposed. Yeast autolysis is carried out. The cell membranes are separated by centrifugation. The autolysate is purified on the gel sulphocationite in the hydrogen form, containing 12-16% divinylbenzene. The resulting peptide aqueous solution is passed sequentially through the gel anion-exchange material to obtain the solution at pH 2.0-2.6, and then through the gel cation-exchange material with the divinylbenzene content of 1-2% or macroporous cation-exchange material.
EFFECT: obtaining highly purified peptides that have biological activity.
11 ex
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Authors
Dates
2015-03-20—Published
2014-04-04—Filed