METHOD OF MEMBRANE ACTIVITY DETERMINATION FOR SUBSTANCES IN LIVE CELLS Russian patent published in 2015 - IPC C12Q1/02 C12N1/10 C12R1/90 

FIELD: biotechnologies.

SUBSTANCE: suspension of Tetrahymena pyriformis cells and test substance is put into detector cells of device for automated calculation of live infusoria number for further detection of live cells per minute, and similar dynamics of live cell number changes in time for compared substances allow for concluding on the equal activity of substances. Calculation methods are based on determination of either infusoria incubation time with the test substance, required for death of half of infusoria, or on cell death rate constant, which can be calculated conveniently by inclination tangent of straight line characterising dependence of moving cell number logarithm on time during observed decline in live cell number. Both values depending on membrane activity characterise membrane activity of test substances and can be used for activity comparison.

EFFECT: membrane activity determination in substances with possible process standardisation.

4 cl, 3 dwg, 1 tbl, 3 ex