METHOD OF DETERMINING FUNCTIONAL ACTIVITY OF HUMAN COMPLEMENT FACTOR B Russian patent published in 2015 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to immunology, and describes a method of determining the functional activity of the human complement factor B by its influence on target cells in a calcium-free medium, which contains magnesium ions, in the presence of the reagent RB, representing human blood serum, selectively deprived of the factor B activity, as the target cells selected are infusoria Tetrahymena pyriformis, the suspension of which together with the tested sample, which contains the determined factor B, and the reagent RB is introduced in measuring cells of the device for the automated calculation of the number of alive infusoria with the following determination of the number of alive cells in each minute, and the concurrence of dynamics of the change in the number of alive cells in time for the tested sample and control one, which represents a pool of 10 serums of healthy donors, supposes the equality of activities of the complement factor B in the said samples.

EFFECT: invention ensures the more accessible target for the action of the complement which infusoria Tetrahymena pyriformis represent.

2 cl, 1 dwg, 1 ex