METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYBRID PROTEIN E7-HSP70 (VERSIONS) Russian patent published in 2015 - IPC C12P21/02 C12N1/19 C07K14/25 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to versions of a method for microbiological synthesis of hybrid protein E7-HSP70. Synthesis of protein E7(6)-HSP70, E7(11)-HSP70, E7(16)-HSP70 or E7(18)-HSP70 is carried out by cultivating the respective yeast strain Saccharomyces cerevisiae VKPM Y-3919, yeast strain Saccharomyces cerevisiae VKPM Y-3853, yeast strain Saccharomyces cerevisiae VKPM Y-4057 or yeast strain Saccharomyces cerevisiae VKPM Y-4058 in suitable conditions in a medium containing saccharose as a carbon source. The cultivation process is carried out in two phases. The first phase is carried out at 25-26°C and initial saccharose concentration of 25-30 g/l. Upon achieving saccharose concentration in the medium of 15 mg/l, the second phase of the cultivation process is carried out, where temperature is lowered and kept not higher than 23°C, pH is kept at 5.7-5.9, and saccharose concentration is maintained via exponential replenishment at 15 mg/l.

EFFECT: group of inventions enables to obtain the end product in amount of not less than 550 mg/l and enables to carry out the cultivation process for not more than 65 hours.

4 cl, 12 dwg, 4 ex