METHOD OF QUANTITATIVE EVALUATION OF BIOFILM FORMATION OF MICROORGANISMS Russian patent published in 2015 - IPC C12Q1/06 G01N13/00 C12R1/385 C12R1/445 

FIELD: biotechnology.

SUBSTANCE: microbial suspension is placed to the sterile Petri dishes prepared for inoculation in with nutrient broth and two agar plates. The Petri dishes with inoculations are incubated at 37°C. After incubation, the plates with the grown biofilm are removed from the culture liquid, are washed with sterile distilled water from planktonic cells and dried in a thermostat. Angles of moistening are measured in 3 and 9 hours. According to the changes in the edge angle of moistening, the specific rate of biofilm formation is determined. At that the specific rate of biofilm formation is calculated by the formula: $${\mu }_b=\frac{1}{t_2-t_1}ln\left(\frac{{\theta }_1}{{\theta }_2}\right),$$ where µ_b is specific rate of biofilm formation, h^-1; t₁ and t₂ is incubation duration, h (3 and 9 hours); θ_1,2 is edge angles of moistening (°), measured after incubation for 3 and 9 hours.

EFFECT: invention enables to speed up and simplify the process of quantitative evaluation of microbial biofilm formation and to increase sensitivity of the method.

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