FIELD: medicine, medicinal microbiology.
SUBSTANCE: method involves growing microorganism culture to be studied in solid nutrient medium followed by preparing microbial suspension and its incubation in the presence of lactoferrin. Control sample is prepared in parallel series. Control and experimental samples are incubated, supernatant is removed from bacterial cells and lactoferrin concentration is determined in supernatant of experimental and control sample by immunoenzyme analysis. Then anti-lactoferrin activity is calculated by difference of concentrations of residual lactoferrin in experimental and control samples. This method provides enhancing the sensitivity and precision in carrying out the quantitative evaluation of anti-lactoferrin activity in broad spectrum of microorganisms that is urgent in diagnosis and prognosis of diseases with bacterial etiology. Invention can be used in determination of persistent indices of microorganisms for assay of their etiological significance in pathological processes.
EFFECT: improved assay method.
3 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETERMINATION OF ANTILACTOFERRIN ACTIVITY IN MICROORGANISMS | 1999 |
|
RU2156807C2 |
| METHOD FOR PREDICTING RECOVALESCENCE SALMONELLOSIS BACTERIOCARRIAGE | 2003 |
|
RU2242756C2 |
| METHOD FOR DIFFERENTIATION OF CULTURES OF Candida albicans OF VAGINAL HABITAT OF WOMEN AT NORMAL AND PATHOGENIC MICROFLORA | 2015 |
|
RU2595370C2 |
| METHOD FOR ASSAY OF ANTI-IMMUNOGLOBULIN ACTIVITY OF MICROORGANISMS | 2002 |
|
RU2236465C2 |
| METHOD FOR PREDICTING SUBCLINICAL MASTITIS FORM OF MICROBIAL ETIOLOGY IN COWS | 2004 |
|
RU2264171C1 |
| METHOD OF DETERMINATION OF ANTIPLATELET CATIONE-PROTEIN ACTIVITY OF MICROORGANISMS | 1997 |
|
RU2120999C1 |
| METHOD FOR DETERMINATION OF ANTI-HEMOGLOBIN ACTIVITY OF MICROORGANISMS | 2004 |
|
RU2262705C2 |
| METHOD OF DETERMINING COLONIZATION RESISTANCE OF HUMAN BODY ECOLOGIC NICHE | 2000 |
|
RU2175673C1 |
| METHOD FOR SELECTING INDIGENOUS STRAINS OF HUMAN INTESTINAL BIFIDOBACTERIA FOR THEIR INCLUSION IN PROBIOTIC PREPARATIONS | 2023 |
|
RU2806579C2 |
| METHOD OF ASSAY OF ANTIHISTONE ACTIVITY OF MICROORGANISMS | 2001 |
|
RU2203956C2 |
