METHOD OF DETERMINING C3-CONVERTASE STABILISATION OF CLASSICAL WAY OF HUMAN COMPLEMENT ACTIVATION Russian patent published in 2015 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: invention relates to laboratory diagnostics, namely to clinical immunology. Method of determining C3-convertase stabilisation of classical way of human complement activation is realised by carrying out reaction of lysis of ram erythrocytes with 0.8% of human blood serum for 10 min. Reaction is stopped by addition of buffer, which contains 10 mM ethylendiamintetraacetic acid, degree of lysis of erythrocytes in control sample is determined. Experimental sample is additionally incubated for 30 min at 37°C, degree of lysis is determined, activity of C3-convertase is calculated as difference between experimental and control samples, with difference higher than 10% it is evaluated as autoimmune pathological condition.

EFFECT: application of simple and fast in realisation method makes it possible to detect preclinical condition of immunodeficiency, can serve as specific marker for immunomodulating therapy, carry out profound research of pathogenesis of autoimmune diseases.

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