SCREENING TEST FOR DETERMINATION OF THE FUNCTIONAL ACTIVITY OF THE RAT COMPLEMENT SYSTEM Russian patent published in 2022 - IPC C12Q1/68 G01N33/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, namely to a method for determining the functional activity of the rat complement system. A method for determining the functional activity of the rat complement system includes carrying out a lysis reaction of human erythrocytes of group A of the AB0 system [E(A)], sensitized with anti-A monoclonal IgM antibodies [E(A)mAb], and serum under conditions of veronal saline buffer (VBS²⁺), incubation of the obtained sample, then evaluate the reaction of complement-dependent lysis of E(A) mAb.

EFFECT: above method improves the accuracy of the method for studying the functional activity of the classical pathway of the complement system, as well as simplifies the registration of the results of the analysis of the functional activity of the classical pathway of the complement system in routine studies and adapts the method for screening studies in clinical diagnostic laboratories.

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