METHOD OF DETERMINING THE ACTIVITY OF THE CLASSICAL AND ALTERNATIVE PATHWAYS OF THE MOUSE COMPLEMENT SYSTEM Russian patent published in 2023 - IPC G01N33/50 G01N33/53 

FIELD: biotechnology.

SUBSTANCE: method is proposed for determining the activity of the classical and alternative complement pathways in the blood serum of mice, including the lysis reaction of 0.25% suspension of rabbit erythrocytes sensitized with human antibodies (EcrAT), 12.5% mouse serum at 37.0±0.5°C, construction of a calibration graph of the dependence of the optical density of A₆₂₀ suspension on the lysis of rabbit erythrocytes, which determines the degree of EcrAT lysis. The total complement activity is determined under VBS²⁺ conditions and incubated for 10 min, the activity of the alternative complement pathway is determined at the same ratios in the hemolytic system only under VBS-Mg²⁺ -EGT conditions and 30 min incubation; mouse classic complement pathway activity is defined as the difference between total complement activity and that of the alternative complement pathway.

EFFECT: invention provides an extension of the arsenal of methods for studying complement-mediated erythrocyte lysis over time.

1 cl, 7 dwg, 2 tbl, 4 ex