KIT FOR LABORATORY DIAGNOSIS OF INFECTIONS CAUSED BY MYCOPLASMA HOMINIS AND UREAPLASMA UREALYTICUM Russian patent published in 2015 - IPC C12Q1/02 

FIELD: biotechnology.

SUBSTANCE: invention is a kit for the laboratory diagnostics of infections caused by urogenital mycoplasmas Mycoplasma hominis and Ureaplasma urealyticum, containing the lyophilized transport medium for the storage of test samples, freeze-dried nutrient medium for detecting Mycoplasma hominis and/or Ureaplasma urealyticum, and strips for identification of Mycoplasma hominis and Ureaplasma urealyticum for the semi-quantitative determining the titer of the pathogen and for determining the sensitivity of Mycoplasma hominis and Ureaplasma urealyticum to antibiotics, which comprises three types of strips, the first of which is designed for identification of Mycoplasma hominis and Ureaplasma urealyticum and for semi-quantitative determining of their titre, and some wells of the first type of strips that are designed for identification and determining the titre of Mycoplasma hominis contain arginine, brilliant blue and clarithromycin, the other part of the wells of this strip that are designed for identification and determining the titre of Ureaplasma urealyticum contain urea and lincomycin, the second type of strips designed for determining the antibiotic susceptibility of Mycoplasma hominis, contains specific reagents for detection of Mycoplasma hominis - arginine, brilliant blue and clarithromycin, and also eight antibiotics - doxycycline, josamycin, midecamycin, ofloxacin, ciprofloxacin, sparfloxacin, moxifloxacin, clindamycin, adsorbed to the wells of the strips in the same concentration, the third type of the strips designed to determine the antibiotic susceptibility of Ureaplasma urealyticum contains specific reagents for detection of Ureaplasma urealyticum - urea and lincomycin, and also eight antibiotics - doxycycline, josamycin, clarithromycin, erythromycin, roxithromycin, azithromycin, ofloxacin and sparfloxacin, adsorbed to the wells of the strips in the same concentration.

EFFECT: use of the kit enables to detect simultaneously Mycoplasma hominis and Ureaplasma urealyticum, to evaluate their titre and to determine the sensitivity to different spectrum of antibiotics, and for Mycoplasma hominis and for Ureaplasma urealyticum different antibiotics are used.

2 dwg, 2 tbl, 3 ex