METHOD OF EXTRACTION OF EXOSOMES FROM BLOOD Russian patent published in 2015 - IPC C12N5/00 

FIELD: biotechnologies.

SUBSTANCE: blood is separated into plasma and cellular fraction. Then the blood cellular fraction is subjected to successive two-phase treatment first by the buffer PBS solution containing 5 mM of EDTA with subsequent centrifugation and collection of supernatant. Cells are treated by equal volume of 0.15-0.35% trypsin solution in PBS with subsequent centrifugation and collection of supernatant. Plasma and extracted supernatants from cellular fraction are merged, cellular debris is removed by centrifugation at 15000-17000 g within 10-20 minutes. Foreign particles of non-exosome origin by filtration through filters with the diameter pores 0.1 mcm, and the total pool of exosomes is settled by ultracentrifugation at 100000-160000 g during 60-120 minutes.

EFFECT: invention allows to increase yield and purity of target product, reduce duration and labour input of the method.

3 cl, 2 dwg, 1 tbl, 3 ex