FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. Method of obtaininf blood exosomes is disclosed, wherein blood is separated into plasma and cellular fraction by centrifugation at 600 g for 20 minutes. Cellular fraction is subjected to successive processing with buffer solution PBS at pH 7.4, incubation for 15 minutes with subsequent centrifugation during 25 minutes at 600 g and collection of first supernatant. It is followed by shaking with subsequent centrifugation for 10 minutes at 500 g and second collection of supernatant. Filtration and ultracentrifugation are performed simultaneously with help of ultracentrifuge filter test tubes. Obtained total pool of blood exosomes includes exosomes of plasma and exosomes associated with surface of blood cells.
EFFECT: invention increases output of end product.
1 cl, 1 tbl, 5 ex
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