FIELD: biotechnology. SUBSTANCE: mammalian cultured cells (in part, Hep G2, COS, BHK tk-t 13,293 and others) are transformed with recombinant expression vector that involves DNA-fragment encoding the matured human factor VII with preceding complete or partial signal sequence. Stably transformed cells are separated and cultured. Then recombinant protein is isolated from the cultured medium. This protein after preliminary activation with suitable protease shows activity in blood coagulation process that is similar with that at effect of natural factor VIIa. EFFECT: increased yield of recombinant form of factor VII, high degree of purity. 3 cl, 8 tbl, 9 ex, 14 dwg
| Title |
Year |
Author |
Number |
| METHOD OF TRANSGENIC SHEEP PRODUCING |
1987 |
- Ehntoni Dzhon Klark[Gb]
- Richard Lejz[Gb]
|
RU2085587C1 |
| RECOMBINATION PLT 9 PLASMIDE DNA WHICH CODES POLYPEPTIDE POSSESSING PROPERTIES OF HUMAN LYMPHOTOXIN, RECOMBINATION PLASMIDE PLT 16 DNA USED FOR CONSTRUCTING PLT 9 DNA, AND 'ESCHERICHIA COLI' BACTERIUM STRAIN - PRODUCER OF POLYPEPTIDE HAVING QUALITIES OF HUMAN LYMPHOTOXIN |
1988 |
- Korobko V.G.
- Dobrynin V.N.
- Filippov S.A.
- Chumakov A.M.
- Panina A.A.
- Boldyreva E.F.
- Nedospasov S.A.
- Shakhov A.N.
- Turetskaja R.L.
|
SU1561510A1 |
| RECOMBINANT PLASMID DNA PTHY 315 ENCODING HYBRID PROTEIN TUMOR NECROSIS α-FACTOR - THYMOSIN-a |
1992 |
|
RU2077586C1 |
| METHOD OF OXYTOCIN PREPARING, RECOMBINANT PLASMID DNA PTOTEILOX-3 ENCODING HYBRID PROTEIN ILOX-3 FOR OXYTOCIN PRODUCING, STRAIN OF ESCHERICHIA COLI - A PRODUCER OF HYBRID PROTEIN ILOX-3 |
1993 |
- Gurevich A.I.
- Kachalina T.A.
- Kajushin A.L.
- Korosteleva M.D.
- Miroshnikov A.I.
- Ovchinnikova T.V.
- Kovalevich T.E.
- Martynova N.Ju.
- Sukhov I.E.
- Khoroshilova N.I.
|
RU2085585C1 |
| RECOMBINANT PLASMID DNA PUR2N ENCODING TICK-BORNE ENCEPHALITIS VIRUS PROTEIN NS1 SHORT FORM AND A METHOD OF ITS CONSTRUCTING |
1989 |
- Pugachev K.V.
- Pletnev A.G.
- Jamshchikov V.F.
- Gorn V.V.
|
SU1822202A1 |
| DNA FRAGMENTS ENCODING HUMAN ERYTHROPOIETIN, RECOMBINANT DNAS, CELL LINE, RECOMBINANT O-GLYCOSYLATED HUMAN ERYTHROPOIETIN, PHARMACEUTICAL COMPOSITION |
1991 |
- Ehdvard Fritch
- Rodni M.Kh'Juvik
- Kennet Dzhekobs
|
RU2120996C1 |
| RECOMBINANT PLASMID PFS 19 DETERMINING SYNTHESIS OF THE SURFACE ANTIGEN S OF HUMAN VIRUS HEPATITIS B |
1992 |
- Sasnauskas Kjastutis Vlado
- Iomantene Rasa Jono
- Janulajtis Arvidas Andrjaus
- Bumjalis Vladas Vlado
- Vetrinas Aurimas Juozo
|
RU2115730C1 |
| RECOMBINANT PLASMID DNA, ENCODING HUMAN FACTOR VII PROTEIN SEQUENCE, CELL LINE BHK/F7, TRANSFORMED WITH PLASMID DNA |
2006 |
- Vorob'Ev Andrej Ivanovich
- Miroshnikov Anatolij Ivanovich
- Gabibov Aleksandr Gabibovich
- Vorob'Ev Ivan Ivanovich
- Ponomarenko Natal'Ja Aleksandrovna
- Shamborant Ol'Ga Georgievna
- Shuster Aleksandr Mikhajlovich
- Mart'Janov Vitalij Afanas'Evich
|
RU2337965C2 |
| RECOMBINANT PLASMID DNA PBGHTRP3, ENCODING CATTLE GROWTH HORMONE, AND THE STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF CATTLE GROWTH HORMONE |
0 |
- Rubtsov Petr Mikhajlovich
- Gorbulev Valentin Grigorevich
- Sverdlova Polina Samojlovna
- Sadiev Sagypash Tulyubekovich
- Shulga Aleksej Anatolevich
- Chupeeva Valentina Vladimirovna
- Chernov Boris Konstantinovich
- Pozmogova Galina Evgenevna
- Golova Yuliya Borisovna
- Skryabin Konstantin Georgievich
- Baev Aleksandr Aleksandrovich
|
SU1708847A1 |
| STRAIN OF RECOMBINANT SMALL POX VIRUS EXPRESSING STRUCTURAL PROTEINS OF THE HORSE VENEZUELAN ENCEPHALOMYELITIS VIRUS AND USEFUL FOR IMMUNOBIOLOGICAL PREPARATIONS PRODUCTION AND A METHOD OF ITS PREPARING |
1993 |
- Frolov I.V.
- Urmanov I.Kh.
- Kolykhalov A.A.
- Netesov S.V.
- Agapov E.V.
- Serpinskij O.I.
- Svjatchenko V.A.
|
RU2091489C1 |
