FIELD: medicine.
SUBSTANCE: method includes the extraction of the total native DNA from skin and hair, specific amplification of the gene 5.8S pPHK Trichophyton verrucosum fragment with the application of primers of the following structure: 5' CCACGATAGGGATCAGCGTT 3', 5' GAAAGTTTTAACTGATTTTGCTTG 3'. Then in case of the electrophoretic detection of the amplification product with the size of 231 bp the presence of a causative agent is determined.
EFFECT: application of the invention makes it possible to determine the fact of a human infection by Trichophyton verrucosum in case of different clinical forms of the disease, including suppressed and atypical ones.
2 ex
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