METHOD AND KIT FOR ENHANCED LABORATORY DIAGNOSIS OF PERTUSSIS INFECTION Russian patent published in 2015 - IPC G01N33/569 

FIELD: medicine.

SUBSTANCE: what is declared is a unified, accelerated, high-sensitive, high-specific, low labour consuming method for enhanced laboratory diagnosis of pertussis infection involving the following stages: taking a pathological material from a posterior wall of the patient's throat, preparing the clinical sample, recovering DNA from the clinical material by sorption method, performing the amplification in the isothermal environment and the electrophoresis in agarose gel, as well as a diagnostic set.

EFFECT: using the group of inventions enables identifying the pertussis agent for 4,5-5 hours from the onset of the examination directly in the patient's material depending on the stage of the disease and in various forms of the clinical course, using the group of inventions also enables establishing the diagnosis of pertussis rapidly in practical healthcare regardless of the disease length and the form of the clinical course for prescribing the timely and adequate therapy, increasing a possibility to examine the infants in pertussis-like diseases and adults with the unapparent clinical pattern, detecting the patients with pertussis in examining the centres of the pertussis infection in patient's environment as soon as possible.

2 cl, 2 ex