FIELD: medicine.
SUBSTANCE: invention is intended for PCR-detection of Atopobium vaginae bacteria, Leptotrichia amnionii, Sneathia sanguinegens and Eggerthella spp. in clinical material. DNA is recovered, amplification and electrophoresis conducted. One performs multiplex PCR using specific primers in cyclic mode. At electrophoretic detection of amplicons with sizes 286 bps, and/or 768 bps, and/or 135 bps, and/or 428 base pairs presence of Atopobium vaginae bacteria, and/or Leptotrichia amnionii and/or Sneathia sanguinegens and/or Eggerthella spp., respectively is detected.
EFFECT: invention ensures simultaneous detection in clinical material up to 4 bacteria types associated with bacterial vaginosis.
1 cl, 1 dwg, 2 tbl, 10 ex
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