COMPOSITIONS AND METHODS OF PRODUCING C5 HYDROCARBON-FREE ISOPRENE UNDER ISOPRENE PRODUCTION AND CELL GROWTH DECOUPLING CONDITIONS AND/OR CONDITIONS OF PRODUCING ISOPRENE AT SAFE OPERATING LEVELS Russian patent published in 2015 - IPC C12N1/20 

FIELD: chemistry.

SUBSTANCE: present invention relates to biotechnology and provides methods of producing isoprene by culturing recombinant cells of fungi or microorganisms. Said cells optionally contain a heterologous isoprene synthase polypeptide and at least one nucleic acid which encodes at least one mevalonate (MVA) pathway polypeptide, such as an acetyl-CoA acetyltransferase polypeptide, 3-hydroxy-3-methylglutaryl-CoA-synthase polypeptide, 3-hydroxy-3-methylglutaryl-CoA-reductase polypeptide, mevalonate kinase polypeptide, phosphomevalonate kinase polypeptide and diphosphomevalonate decarboxylase polypeptide. Said cells produce more than 400 nmol/g_wcm/hr of isoprene.

EFFECT: invention enables to obtain isoprene using a method of culturing said recombinant cells with high output.

31 cl, 111 dwg, 5 tbl, 13 ex