FIELD: biochemistry.
SUBSTANCE: group of inventions relates to biochemistry. Polynucleotide is disclosed, coding protein with isoprenesynthase activity. Protein is disclosed, which has isoprenesynthase activity. Expression vector is also disclosed, containing said polynucleotide or polynucleotide, coding said protein. Host cell for production of isoprenesynthase is disclosed, containing said vector. Method of producing protein with isoprenesynthase activity using above host cell is disclosed. Method of producing isoprene monomer from dimethylallyldiphosphate in presence of said protein or using above host cell in medium with dimethylallyldiphosphate is disclosed. Method of producing isoprene polymer is disclosed involving step of producing isoprene monomer using said method.
EFFECT: group of inventions increases production of isoprene by several times in comparison with production of isoprene using isoprenesynthase produced from kudzu pilar or poplar.
14 cl, 4 dwg, 33 tbl, 16 ex
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