FIELD: biotechnologies.
SUBSTANCE: anti-gene is mixed with the tested blood serum in various dilutions in microplate cavities with V-shaped bottom with subsequent visualization of results of agglutination reaction using the ultra-violet radiation source, mainly the transilluminator. The anti-gene is the fluorescent and labelled suspension of inactivated bacterial cells which is obtained by treatment of bacterial cells by 1.0% water solution of the fluorescent dye selected from the group: bromic etidium, propidium iodide, acridic orange.
EFFECT: invention allows to simplify the immunofermental analysis for identification of existence of various pathogenic microorganisms and to automate the accounting of results.
3 cl, 3 tbl, 4 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR DETECTING BACTERIAL ANTIGENS | 2010 |
|
RU2451083C1 |
METHOD FOR DETECTING INFECTION OF HUMANS AND ANIMALS WITH SARS CoV 2 AND A DIAGNOSTIC KIT FOR IMPLEMENTING THE METHOD | 2021 |
|
RU2776295C1 |
METHOD FOR DIFFERENTIAL DIAGNOSTICS OF YERSINIOSIS AND RESPECTIVE DIAGNOSTIC KIT | 2007 |
|
RU2345365C1 |
METHOD FOR DETECTING BACTERIA OF GENUS AZOSPIRILLUM, WHICH HAVE COMMON ANTIGENIC DETERMINANTS IN COMPOSITION OF LIPOPOLYSACCHARIDES | 2013 |
|
RU2572350C2 |
Stenotrophomonas maltophilia STRAIN USED FOR OBTAINING OF ANTIGEN FOR IDENTIFICATION OF ANTIBODIES TO Stenotrophomonas maltophilia IN BIOLOGICAL MEDIUMS | 2015 |
|
RU2566554C1 |
PASTEURELLA MULTOCIDA "PM - B" BACTERIUM STRAIN FOR MANUFACTURE OF BIOPREPARATIONS FOR SPECIFIC PREVENTION OF PASTEURELLOSIS (HEMORRHAGIC SEPTICEMIA) IN CATTLE CAUSED BY SEROGROUP B PASTEURELLA | 2023 |
|
RU2818959C1 |
Burkholderia cepacia B-7518 STRAIN USED FOR OBTAINING OF ANTIGEN FOR IDENTIFICATION OF ANTIBODIES TO Burkholderia cepacia IN BIOLOGICAL ENVIRONMENTS | 2014 |
|
RU2566555C1 |
DIAGNOSTIC TECHNIQUE FOR RICKETTSIAL DISEASES GROUP OF SPOTTED FEVER, IMMUNOASSAY DIAGNOSTIC TEST SYSTEM FOR IMPLEMENTATION THEREOF | 2019 |
|
RU2726484C1 |
METHOD FOR DIFFERENTIAL DIAGNOSTICS OF PSEUDO-TUBERCULOSIS AND INTESTINAL YERSINIOSIS AND DIAGNOSTIC SET FOR ITS REALISATION | 2007 |
|
RU2339952C1 |
METHOD FOR IMMUNOENZYMOMETRIC DETECTION OF AGENT OF PSEUDOTUBERCULOSIS 1 SEROTYPE ON BASIS OF MONOCLONAL ANTIBODIES TO O-SIDE CHAINS OF LIPOPOLYSACCHARIDE | 2018 |
|
RU2695525C1 |
Authors
Dates
2015-09-27—Published
2014-06-03—Filed